PCR & Real time PCR techniques and its applications
- Basic Microbial techniques and their storage Culturing methods, Microbial isolation techniques, Media optimisation, Storing of Microorganisms
- Basic Microbial and Molecular techniques Microbial culture techniques, Growth curve analysis, Nucleic acid extraction, Electrophoresis separation, Polymerase chain reaction (PCR), Primer designing and standardisation
- Microbial Gene Expression studies Microbial nucleic acid extraction and Quantification, Primer designing and standardisation, Real Time PCR: Gene Expression studies, Result Interpretation
- Human Gene Expression studies Human nucleic acid extraction and Quantification, PCR, Primer designing and standardisation, Result interpretation
Translational Cancer Research Techniques and Molecular Cloning and Cancer Hall-mark research techniques
- Bacterial Cloning Technique
- Media preparation and vector selection
- Bacteria culture and Maintenance
- Preparation of Competent cells
- Restriction digestion
- Ligation and Transformation
- Small scale isolation of plasmid DNA
- Large scale isolation of plasmid DNA
- Restriction digestion
- Ligation
- Gel Electrophoresis
- Elution of DNA from gel
- Expression studies
- Proteomics
- Culture maintenance
- Extraction and estimation of protein
- Purification of protein
- SDS-PAGE
- Staining
- Western Blot
- Western Blot visualization
- Technical Training
- Introduction of basic components of Cell culture and environment
- Media preparation
- Sterile technique and contamination control
- Specific cell types and their requirement
- Culture of cells in attachment and suspension and scaling up of cultures
- Passaging of cells
- Study of invitro cell growth behaviour
- Determination of cell growth
- Cell viability and cell proliferation assay techniques
- Cryopreservation and retrieval of cells from frozen storage
- Functional studies related to Cancer
- Soft agar assay
- Clonogenic assay
- Wound healing assay
- Proliferation assay
- Invasion/Migration assay
- Angiogenesis
- Functional studies related to Cancer
- Soft agar assay
- Clonogenic assay
- Wound healing assay
- Proliferation assay
- Invasion/Migration assay
- Angiogenesis
- DNA damage and repair assays
- DNA damage marker studies by western blot
- DNA fragmentation
- Comet assay
- Pulse field gel electrophoresis
- Halo assay
BIO-COMPUTING
- Drug Designing
- Protein Structure Prediction
- Sequence analysis
- Clinical SAS
- Nucleotide Database Search
- Nucleotide Sequence Retrieval
- Sequence Alignment
- Primer Designing
- Phylogenetic Tree Construction
- Protein Sequence Retrieval
- RASMOL - Protein Structure Visualization tool
- sbdbv - Swiss-PdbViewer
- Motif scan and Identification
- Hex – Docking
- ExPASy
- ISIS Draw – Structure Drawing
- NCBI Literature Search
- Special Techniques
- Cell line revival, culture and storage methods
- Analysis of gene expression across cell lines by semi-quantitative RT-PCR
- Analysis of gene expression across cell lines by quantitative RT-PCR
- Killing curve assay for eukaryotic selection markers
- Transient gene silencing in cell line by liposome mediated SiRNA transfection
- Analysis of the impact of gene expression in human cell line
- Transient exogenous gene expression in human cell line
- Engineering of human cell line to continuously express the gene on interest
- Stable gene silencing by shRNA plasmid transfection/transduction
- Analysis of protein expression in cell lines by Western blotting.
- Gene over expression in human cells by reverse transfection.
- Analysis of signalling pathway activity in human cell lines by dual luciferase reporter assay
- Impact of modulation of gene expression on signalling pathway.
- Analysis of anchorage independent growth property of human cells.
- Determination of in-vitro toxicity of a compound on a call line.
- Investigation of the epigenomic repression of a gene expression in cell line.
- Analysis of cellular differentiation in human cell line.
- Elucidation of the transcriptional regulation of gene promoters by reporter assay.
- Determination of transcription factor-DNA interactions in human cells by Chromatin Immunoprecipitation (ChIP) assay.
- Investigation of the epigenetic modification of a genomic region by bisulfite methylation assay.
- Analysis of cellular apoptosis by flow cytometer/Western blot
- Cell cycle phase analysis of human cell lines by flow cytometer.
- Investigation of the cellular localization of the protein of interest by immunostaning.
- What next after U.G / P.G?
- Secrets behind Smart work
- CSIR/UGC/NET/ICMR/ICAR
- TOEFL / GRE / IELTS Preparation
- CV/ Resume Preparation
- Hints to face an Interview